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diaphot 300 inverted microscope  (Nikon)


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    Structured Review

    Nikon diaphot 300 inverted microscope
    Diaphot 300 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diaphot+microscope/Objectives/pm41569431-58-12-11
    Average 99 stars, based on 59714 article reviews
    diaphot 300 inverted microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer
    Article Snippet: Coverslips were applied with ProLong® Gold Antifade Reagent and DAPI (Life Technologies). .. Imaging was performed on a Nikon DiaPhot microscope with digital camera and NIS-Elements 4.11.00 (Nikon Instruments Inc., Melville, NY). ..

    Article Title: Deletion of NADPH oxidase 2 in chondrocytes exacerbates ethanol-mediated growth plate disruption in mice without major effects on bone architecture or gene expression.
    Article Snippet: .. The average numbers of cells in a ×40 magnification were enumerated in three separate fields from at least three individual mice per group in a blinded fashion using A Nikon DIAPHOT microscope and imaging software (Nikon Instruments Inc.). ..

    Microscopy:

    Article Title: Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer
    Article Snippet: Coverslips were applied with ProLong® Gold Antifade Reagent and DAPI (Life Technologies). .. Imaging was performed on a Nikon DiaPhot microscope with digital camera and NIS-Elements 4.11.00 (Nikon Instruments Inc., Melville, NY). ..

    Article Title: Spheroid architecture strongly enhances miR-221/222 expression and promotes oxidative phosphorylation in an ovarian cancer cell line through a mechanism that includes restriction of miR-9 expression.
    Article Snippet: Figs.. Figures JG-B Janus green B miR MicroRNA N-cad N-cadherin OXPHOS Oxidative phosphorylation PBS Phosphate-buffered saline, PH 7.4 Pyrmalate Pyruvate + malate qRT-PCR Quantitative reverse transcriptase polymerase chain reaction RPMI Rosewell Park Memorial Institute 1640 medium Sus Suspension TCP Tissue culture polystyrene ULP Ultralow attachment plastic Abbreviations Adh Adherent CSC Cancer stem cells DMEM Dulbecco’s Modified Eagle’s medium Fig.. Figure

    Article Title: The MCU and MCUb amino-terminal domains tightly interact: mechanisms for low conductance assembly of the mitochondrial calcium uniporter complex
    Article Snippet: Transfection of 1 μg of the resultant pEGFP-MCU-GCaMP6f, -MCUb-GCaMP6f or -MCUb-ΔNTD-GCaMP6f was done with PolyJet (SignaGen). .. Approximately 24 h post-transfection, the dish was visualized on a Nikon Diaphot microscope equipped with a 40× objective (Nikon Fluor 40/1.30 Oil Ph4) to locate a cluster of ∼2–5 GCaMP6f-expressing cells. .. Fluorescent excitation and emission intensity detection was driven through a Felix Gx software (version 4.9)-controlled PTI RatioMaster RM50 system equipped with a D104 photometer (Horiba).

    Article Title: CPX, a selective A 1 -adenosine-receptor antagonist, regulates intracellular pH in cystic fibrosis cells
    Article Snippet: .. Confluent cell monolayers in sample chambers were loaded for 60 min at 25°C with either the pH-sensitive fluorescent dye BCECF by incubation with BCECF-AM (6 μM in PSS containing 0.01% BSA and 10 FM probenecid) or with the Ca 2+ -sensitive fluorescent dye fura 2 by incubation with fura 2-AM (2 μM in PSS containing 0.01% BSA), then washed twice and kept in PSS until use. pH i and [Ca 2+ ] i were monitored in single cells within a confluent monolayer at 25°C using an ARCM-MIC microfluorimeter (Spex Industries, Edison, NJ) coupled to a Nikon Diaphot microscope (Nikon, Tokyo, Japan) via a fiber-optic cable. ..

    Article Title: Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes
    Article Snippet: .. We observed the cells using a using a Nikon Diaphot microscope with a 40x NA 1.3 or 100x NA 1.3 phase-contrast objective. ..

    Article Title: Deletion of NADPH oxidase 2 in chondrocytes exacerbates ethanol-mediated growth plate disruption in mice without major effects on bone architecture or gene expression.
    Article Snippet: .. The average numbers of cells in a ×40 magnification were enumerated in three separate fields from at least three individual mice per group in a blinded fashion using A Nikon DIAPHOT microscope and imaging software (Nikon Instruments Inc.). ..

    Article Title: Analysis of the levels of lysine-specific demethylase 1 ( LSD1 ) mRNA in human ovarian tumors and the effects of chemical LSD1 inhibitors in ovarian cancer cell lines
    Article Snippet: HRP signal was detected by ECL (Amersham-GE, Pittsburgh, PA, USA) and autoradiography film. .. Bright-field images were taken in a Nikon Diaphot microscope at a 10x magnification. .. Image acquisition was performed with a Canon Digital Rebel XTi camera, using ZoomBrowser EX5.7 and EOS Utility software (Canon) for remote picture shooting.

    Article Title: Pneumocystis cell wall β-glucan stimulates calcium-dependent signaling of IL-8 secretion by human airway epithelial cells
    Article Snippet: Fluorescence excitation, image acquisition, and Ca 2+ data analyses were controlled using a dedicated video fluorescence imaging system (Metafluor; Universal Imaging Corporation). .. Cells were imaged using an inverted Nikon Diaphot microscope equipped with a Nikon Fluor X20 objective lens. .. Fura 2-loaded cells were alternately excited at 340 and 380 nm using a Lambda 10-2 filter changer (Sutter Instrument Company).

    Cell Culture:

    Article Title: Spheroid architecture strongly enhances miR-221/222 expression and promotes oxidative phosphorylation in an ovarian cancer cell line through a mechanism that includes restriction of miR-9 expression.
    Article Snippet: Figs.. Figures JG-B Janus green B miR MicroRNA N-cad N-cadherin OXPHOS Oxidative phosphorylation PBS Phosphate-buffered saline, PH 7.4 Pyrmalate Pyruvate + malate qRT-PCR Quantitative reverse transcriptase polymerase chain reaction RPMI Rosewell Park Memorial Institute 1640 medium Sus Suspension TCP Tissue culture polystyrene ULP Ultralow attachment plastic Abbreviations Adh Adherent CSC Cancer stem cells DMEM Dulbecco’s Modified Eagle’s medium Fig.. Figure

    Incubation:

    Article Title: CPX, a selective A 1 -adenosine-receptor antagonist, regulates intracellular pH in cystic fibrosis cells
    Article Snippet: .. Confluent cell monolayers in sample chambers were loaded for 60 min at 25°C with either the pH-sensitive fluorescent dye BCECF by incubation with BCECF-AM (6 μM in PSS containing 0.01% BSA and 10 FM probenecid) or with the Ca 2+ -sensitive fluorescent dye fura 2 by incubation with fura 2-AM (2 μM in PSS containing 0.01% BSA), then washed twice and kept in PSS until use. pH i and [Ca 2+ ] i were monitored in single cells within a confluent monolayer at 25°C using an ARCM-MIC microfluorimeter (Spex Industries, Edison, NJ) coupled to a Nikon Diaphot microscope (Nikon, Tokyo, Japan) via a fiber-optic cable. ..

    Software:

    Article Title: Deletion of NADPH oxidase 2 in chondrocytes exacerbates ethanol-mediated growth plate disruption in mice without major effects on bone architecture or gene expression.
    Article Snippet: .. The average numbers of cells in a ×40 magnification were enumerated in three separate fields from at least three individual mice per group in a blinded fashion using A Nikon DIAPHOT microscope and imaging software (Nikon Instruments Inc.). ..



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